Senior Associate / Principal Associate Scientist, Gene Editing
Company
Generation Bio
Location
Cambridge
Type
Full Time
Job Description
Generation Bio is innovating genetic medicines to provide durable, redosable treatments for people living with rare and prevalent diseases. The company’s non-viral platform incorporates a novel DNA construct called closed-ended DNA, or ceDNA; a unique cell-targeted lipid nanoparticle delivery system, or ctLNP; and a highly scalable capsid-free manufacturing process that uses its proprietary cell-free rapid enzymatic synthesis, or RES, to produce ceDNA. The platform is designed to enable multi-year durability from a single dose, to deliver large genetic payloads, including multiple genes, to specific tissues, and to allow titration and redosing to adjust or extend expression levels in each patient. RES has the potential to expand Generation Bio’s manufacturing scale to hundreds of millions of doses to support its mission to extend the reach of genetic medicine to more people, living with more diseases, around the world.
We are a thriving, collaborative, creative community of 110 people with diverse talents, beliefs, and experiences headquartered in Cambridge, Massachusetts.
Delve further. Please visit www.generationbio.com or follow @generationbio. To see additional roles, please also check out https://generationbio.com/careers/
SUMMARY:
Generation Bio is seeking an energetic and highly motivated molecular biologist with broad expertise in engineering and development of CRISPR/ Cas systems for therapeutic application. The Scientist hired will play a key role in the development of editing technology to support our in vivo sickle cell editing program.
The Scientist will plan and execute experiments to optimize and engineer CRISPR/ Cas systems to meet program goals. Through application of synthetic biology techniques, rationale design and genetic screens the successful candidate will develop novel editing technology. This role is very cross functional with key interactions between bioinformatics, cell biology, pharmacology & formulation teams.
RESPONSIBILITIES:
- Support execution of editing testing pipeline in HSCs (Design, transfection, analysis of editing outcomes)
- Perform screening (Rationale and unbiased) to optimize nuclease and guide for increased performance (on/ off target profile)
- Assist development of technical strategy in collaboration with broader team
- Work independently with guidance and support from Manager and broader molecular biology team
- Work with cross functional team including pharmacology, cell biology and bioinformatics
QUALIFICATIONS:
- Bachelors or Master of science in Molecular Biology, Biochemistry, Microbiology or related field
- A minimum of 2 years of industry experience in relevant field is preferred.
- Fluency and extensive hands-on experience with molecular biology and synthetic biology techniques with a focus on application to mammalian systems
- Experience executing optimizing/ engineering CRISPR/ Cas systems (nuclease and guide) is preferred
- Expertise with mammalian cell culture is desirable, especially Primary cells (Eg. HSCs)
- Experience and knowledge of multiple gene-editing systems required
- Experience with experimental and bioinformatic analysis of editing outcomes (on and off target) is preferred.
POSITION: Full-Time, Exempt
EEOC Statement: Generation Bio believes in a diverse environment and is committed to equal employment opportunity for all its employees and qualified applicants. We do not discriminate in recruitment, hiring, training, promotion or any other employment practices for reasons of race (to include traits historically associated with race including, but not limited to, hair texture, hair type, hair length, and protective hairstyles such as braids, locks, twists, hair coverings, and Bantu knots), color, gender, religion, national origin, gender identity, age, sexual orientation, marital or veteran status, disability, or any other legally protected status. Generation Bio will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.
Date Posted
03/07/2024
Views
3
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