Scientist I/II, Integrative Biology

About Sail:

The Role:

We are seeking to hire an innovative and highly motivated Scientist I/ II to join the Integrative Biology group at Sail Biomedicines. Integrative Biology is a dynamic, expanding, and collaborative team working to establish foundational data sets supporting the development of Sail’s core technological platform and novel therapeutic applications. The successful candidate will be a key member of this cross-functional team of molecular biologists, immunologists, formulation scientists, and AI/ML engineers evaluating lipid nanoparticle formulations and the optimization of RNA cargo. A successful candidate thrives in a fast-paced, results-oriented environment managing several projects in parallel. The ideal candidate will have a diverse biology background that combines significant experience in ex vivo and cell-based biology, cell targeting, immune disease and/or vaccine biology.  

Responsibilities:

• Assay development for the evaluation promising lipid nanoparticle formulations and mRNA cargoes using both in vitro and ex vivo techniques 
• Lead flow cytometry efforts for the evaluation of immune and non-immune cells  
• Routine performance of a variety of in vitro biology assays including but not limited to cellular uptake, protein expression, and immune cell engagement 
• Plan in vivo studies to be performed in mice and rats
• Assist in murine tissue isolation and processing, including homogenization and generation of single cell suspensions for downstream analysis
• Work collaboratively with the computational team in the creation of structured data sets and the build-out of a robust AI/ML engine
• Take on projects outside of existing expertise from planning through data analysis
• Manage multiple experiments, projects, and interactions with supporting functions
• Interpret, troubleshoot, and present complex datasets to diverse audiences 

Necessary Qualifications: 

• PhD. in the Biological Sciences or closely related field with 2+ years of experience working in either industry or academic research setting (equivalent combination of education and experience) 
• Skilled in all aspects of flow cytometry daily activities, including ex vivo tissue processing, cell staining, acquisition, and machine maintenance
• Expertise in multiparameter (>10-color) flow cytometry analysis, compensation, and gating strategies for analysis of mixed cell populations
• Proficient with the culture of primary human cells and cell-based assays
• Competent in molecular biology techniques including PCR, qRT-PCR, and digital PCR
• Excellent documentation, organizational and communication skills
• Able to work effectively with minimal direction and oversight
• Dedicated to contributing to shared project and organizational goals working in a matrixed team  

Desired Qualifications:  

• Experience working with RNA and/or lipid nanoparticles
• Knowledge and experience in biodistribution, vaccine, and immune-oncology a plus 
• Hands-on experience using the Cytek Aurora and spectral flow cytometry  
• Ability to independently perform plate-based assays ELISA, MSD, Luminex, and/or ELISPOT