Scientist I/II - Therapeutic Genome Editing - Neuromuscular

KEY RESPONSIBILITIES

• Execution of molecular biology, cloning, cell culture, genome editing, transfection, viral transduction and a broad range of functional assays
• Design and execute experiments to develop, refine, and validate in vitro and in vivo CRISPR systems in muscle in vitro system and tissues
• Apply advanced techniques in molecular biology, cellular biology, and biochemistry to demonstrate CRISPR efficacy and tolerability in muscle in vitro and in vivo systems
• Collaborate closely with cross-functional teams including molecular biologists, bioinformaticians, and biologists to ensure the successful translation of research findings into actionable development strategies
• Provide technical support for phenotyping and analytical characterization of cellular or animal models and genome editing outcomes
• Accurately and timely record, document, analyze and communicate laboratory data to senior staff and team members
• Conducts all activities in a safe and efficient manner, and adheres to site environmental health and safety (EHS) requirements
• Other duties as assigned 

REQUIRED QUALIFICATIONS

• Bachelor’s degree in molecular and cell biology, immunology, biochemistry, or a related field and 5 years relevant industry experience or Masters degree and 3 years experience or PhD in molecular and cell biology, immunology, biochemistry, or a related field (Scientist I)
• Bachelor’s degree in molecular and cell biology, immunology, biochemistry, or a related field and 6 years relevant industry experience or Masters degree and 4 years experience or PhD and at least 1 year of industry experience (Scientist II) 
• Proficiency in mammalian cell culture, transfections, viral transductions, molecular cloning, PCR, genomic DNA and mRNA purification, cDNA library prep, qPCR, ddPCR, NGS library prep, ELISA, western blot

PREFERRED QUALIFICATIONS

• 5+ years industry work experience 
• Master’s degree
• Experience working with AAV and/or LNP
• Experience with NGS sample preparation
• Experience with complex cell culture systems (cardiomyocytes, myoblasts, myotubes) or patient derived cells (iPSC, fibroblasts)
• Experience developing human therapies using genome editing systems
• Knowledge of Microsoft Office Suite and other relevant data analysis software (Flowjo, GraphPad Prism, etc.)
• Strong organizational and record-keeping skills, excellent interpersonal, verbal and written communication skills.
• Highly motivated with ability to work efficiently and productively in a fast-paced highly collaborative team environment

BENEFITS

• Company-paid health/vision/dental benefits
• Unlimited vacation and generous sick time
• Company-sponsored meals and snacks
• Wellness, caregiver and ergonomics benefits
• 401(k) with company matching